Anthocyanin-rich fractions from red raspberries attenuate inflammation both in vitro and in vivo.

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Abstract Title:

Anthocyanin-rich fractions from red raspberries attenuate inflammation in both RAW264.7 macrophages and a mouse model of colitis.

Abstract Source:

Sci Rep. 2014 ;4:6234. Epub 2014 Aug 29. PMID: 25167935

Abstract Author(s):

Li Li, Liyan Wang, Zhiqin Wu, Lijun Yao, Yonghou Wu, Lian Huang, Kan Liu, Xiang Zhou, Deming Gou

Article Affiliation:

Li Li

Abstract:

Edible berries have a broad spectrum of biomedical functions, including improving immune responses and reducing risk for chronic diseases. In this study, the anti-inflammatory activities of crude extracts (CEs), anthocyanin-rich fractions (ARFs), and des-anthocyanin fractions (DAFs) from seven berries were evaluated based on their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)/IFN-γ-activated RAW264.7 macrophages. ARFs from red raspberries (RR-ARFs) exhibited the highest efficiency in suppressing NO synthesis. The anti-inflammatory properties were also demonstrated by reducing the expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1 beta (IL-1β) and IL-6 in RAW264.7 cells. The luciferase reporter assay demonstrated that the activities of NF-κB and AP-1 signaling pathways were significantly suppressed by RR-ARFs. Further studies showed that RR-ARFs decreased the phosphorylation of IKK, IκBα, p65 and JNK and the nucleartranslocation of p65 in LPS/IFN-γ-stimulated RAW264.7 cells. In a mouse colitis model, dextran sulfate sodium (DSS)-induced weight loss and histological damage were significantly ameliorated by RR-ARFs treatment. Taken together, our results indicate that RR-ARFs attenuate inflammation both in vitro and in vivo primarily by inhibiting the activation of NF-κB and MAPKs. The anti-inflammatory of RR-ARFs could be harnessed and applied in animal agriculture, drug and food industries.

Academic Cite:

Li Li, Liyan Wang, Zhiqin Wu, Lijun Yao, Yonghou Wu, Lian Huang, Kan Liu, Xiang Zhou, Deming Gou. Anthocyanin-rich fractions from red raspberries attenuate inflammation in both RAW264.7 macrophages and a mouse model of colitis. Sci Rep. 2014 ;4:6234. Epub 2014 Aug 29.

Pubmed-Cite:

Li Li, Liyan Wang, Zhiqin Wu, Lijun Yao, Yonghou Wu, Lian Huang, Kan Liu, Xiang Zhou, Deming Gou. Anthocyanin-rich fractions from red raspberries attenuate inflammation in both RAW264.7 macrophages and a mouse model of colitis. Sci Rep. 2014 ;4:6234. Epub 2014 Aug 29. PMID: 25167935

GMI-Cite:

Li Li, Liyan Wang, Zhiqin Wu, Lijun Yao, Yonghou Wu, Lian Huang, Kan Liu, Xiang Zhou, Deming Gou. Anthocyanin-rich fractions from red raspberries attenuate inflammation in both RAW264.7 macrophages and a mouse model of colitis. Sci Rep. 2014 ;4:6234. Epub 2014 Aug 29. PMID: 25167935

Article Published Date : Dec 31, 2013

Study Type : Animal Study, In Vitro Study

Additional Links

Substances : Anthocyanins : CK(372) : AC(136), Raspberry : CK(47) : AC(32)

Diseases : Colitis : CK(255) : AC(111), Inflammation : CK(3240) : AC(882), Lipopolysaccharide-Induced Toxicity : CK(380) : AC(218)

Pharmacological Actions : Anti-Inflammatory Agents : CK(4861) : AC(1630), Cyclooxygenase 2 Inhibitors : CK(464) : AC(272), Interleukin-1 beta downregulation : CK(478) : AC(205), Interleukin-6 Downregulation : CK(1137) : AC(354), NF-kappaB Inhibitor : CK(1114) : AC(694)

Additional Keywords : Plant Extracts : CK(7645) : AC(2539)