“Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway.”–
– GreenMedInfo Summary
Abstract Title:
Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway.
Abstract Source:
Oncol Rep. 2013 Feb ;29(2):571-8. Epub 2012 Dec 4. PMID: 23232982
Abstract Author(s):
Lei Peng, An Liu, Yue Shen, Hua-Zi Xu, Shi-Zhou Yang, Xiao-Zhou Ying, Wei Liao, Hai-Xiao Liu, Zhong-Qin Lin, Qing-Yu Chen, Shao-Wen Cheng, Wei-Dong Shen
Article Affiliation:
Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325000, P.R. China.
Abstract:
Thymoquinone (TQ), the predominant bioactive constituent derived from the medicinal spice Nigella sativa (also known as black cumin), has been applied for medical purposes for more than 2,000 years. Recent studies reported that thymoquinone exhibited inhibitory effects on the cell proliferation of several cancer cell lines. This study was performed to investigate the antitumor and anti-angiogenic effects of thymoquinone on osteosarcoma in vitro and in vivo. Our results showed that thymoquinone induced a higher percentage of growth inhibition and apoptosis in the human osteosarcoma cell line SaOS-2 compared to that of control, and thymoquinone significantly blocked human umbilical vein endothelial cell (HUVEC) tube formation in a dose-dependent manner. To investigate the possible mechanisms involved in these events, we performed electrophoretic mobility shift assay (EMSA) and western blot analysis, and found that thymoquinone significantly downregulated NF-κB DNA-binding activity,XIAP, survivin and VEGF in SaOS-2 cells. Moreover, the expression of cleaved caspase-3 and Smac were upregulated in SaOS-2 cells after treatment with thymoquinone. In addition to these in vitro results, we also found that thymoquinone inhibits tumor angiogenesis and tumor growth through suppressingNF-κB and its regulated molecules. Collectively, our results demonstrate that thymoquinone effectively inhibits tumor growth and angiogenesis both in vitro and in vivo. Moreover, inhibition of NF-κB and downstream effector molecules is a possible underlying mechanism of the antitumor and anti-angiogenic activity of thymoquinone in osteosarcoma.
Academic Cite:
Lei Peng, An Liu, Yue Shen, Hua-Zi Xu, Shi-Zhou Yang, Xiao-Zhou Ying, Wei Liao, Hai-Xiao Liu, Zhong-Qin Lin, Qing-Yu Chen, Shao-Wen Cheng, Wei-Dong Shen. Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway. Oncol Rep. 2013 Feb ;29(2):571-8. Epub 2012 Dec 4.
Pubmed-Cite:
Lei Peng, An Liu, Yue Shen, Hua-Zi Xu, Shi-Zhou Yang, Xiao-Zhou Ying, Wei Liao, Hai-Xiao Liu, Zhong-Qin Lin, Qing-Yu Chen, Shao-Wen Cheng, Wei-Dong Shen. Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway. Oncol Rep. 2013 Feb ;29(2):571-8. Epub 2012 Dec 4. PMID: 23232982
GMI-Cite:
Lei Peng, An Liu, Yue Shen, Hua-Zi Xu, Shi-Zhou Yang, Xiao-Zhou Ying, Wei Liao, Hai-Xiao Liu, Zhong-Qin Lin, Qing-Yu Chen, Shao-Wen Cheng, Wei-Dong Shen. Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway. Oncol Rep. 2013 Feb ;29(2):571-8. Epub 2012 Dec 4. PMID: 23232982
Article Published Date : Jan 31, 2013
Study Type : In Vitro Study
Additional Links
Substances : Thymoquinone : CK(386) : AC(227)
Diseases : Osteosarcoma : CK(141) : AC(72)
Pharmacological Actions : Anti-Angiogenic : CK(197) : AC(137), NF-kappaB Inhibitor : CK(1114) : AC(694)
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